Columns
New application note: AEX-MS - a powerful tool for charge heterogeneity characterisation of an IgG4-based mAb
Feb 19 2024
The efficacy and safety of mAbs is ensured by tightly controlled critical quality attributes (CQA) such as the charge heterogeneity. To monitor the CQAs of mAbs, sensitive and reliable analytical methods are essential. This application note demonstrates the successful charge heterogeneity analysis of an IgG4-based mAb with a pI of 6.6 using a strong anion exchange column, BioPro IEX QF, coupled to mass spectrometry (MS) detection. PNGase F-mediated deglycosylation enhanced the resolution of glycosylated variants, allowing for precise monitoring of Fc CQAs.
Download the application note.
More information online
Digital Edition
Lab Asia 31.6 Dec 2024
December 2024
Chromatography Articles - Sustainable chromatography: Embracing software for greener methods Mass Spectrometry & Spectroscopy Articles - Solving industry challenges for phosphorus containi...
View all digital editions
Events
Nov 27 2024 Istanbul, Turkey
Jan 22 2025 Tokyo, Japan
Jan 22 2025 Birmingham, UK
Jan 25 2025 San Diego, CA, USA
Jan 27 2025 Dubai, UAE