System for Direct Ionisation Analysis of Biological Samples

Combining proprietary Laser Ablation Electrospray Ionisation (LAESI) technology with a high performance and versatile XYZ platform, the LAESI DP-1000 system from Protea Biosciences Inc allows fast and accurate direct ionisation and mapping of biomolecules in biological samples and open well plates.  Designed for 2D and 3D sample imaging, the LAESI DP-1000 enables mass spectrometric analysis of any sample containing water without the need for any additional sample preparation. In a two-stage process, ambient pressure laser ablation and subsequent electrospray ionisation allow mass spectrometric analysis of living cells and tissues, without the introduction of artifacts or sample degradation. Tissues are elegantly mapped in two and three dimensions, displaying the molecular profile across the sample, or profiling the spatial distribution of intracellular molecules throughout the tissue.

The LAESI DP-1000 facilitates the imaging of open well plates to enable high-throughput biological fluid

analysis. Biological fluids can be directly analysed for compound detection or quantitative studies without

any upstream sample preparation. Samples can be analysed in a variety of standard well plate sizes, with ultra-fast

programmable stage movement to increase laboratory productivity and overall sample processing capabilities.

The LAESI DP-1000 conveniently integrates with different mass spectrometers, including single quad, triple quad, TOF and HDMS systems from several manufactures. This integration capitalises on high resolution, accurate mass and quantitative aspects of a coupled mass spec system. Fully adjustable vertical and horizontal articulations of the LAESI DP-1000 easily adjust the system to fit various bench heights and laboratory set-ups.