Multiplex NIR Imaging of Protein Blots

The BioSpectrum® Imaging System from UVP is configured for use in multiple imaging applications. The BioSpectrum combined with the BioLite™ MultiSpectral Light Source provides a powerful system to excite and illuminate wavelengths for applications including multiplex NIR imaging. The BioSpectrum System supplies not only a full range of wavelengths for excitation, also enables rapid, high resolution image capture through the use of cooled CCD camera and low light lenses. Exposures times are very fast, much faster than laser scanning.

The BioSpectrum Imaging System with BioLite MultiSpectral Source features: easy to use VisionWorks®LS software automates research with templates for consistent, reproducible results; high resolution and low light sensitivity cooled CCD camera and optics allow quick detection of a full range of visible and NIR fluorescent dyes; automated imaging of white light and multiple different fluorescent tags on a single blot; and a combination of up to 8 excitation and 5 emission wavelengths for meet all spectral ranges in single experiment.

With the extensive range of excitation and emission filters available, researchers can detect as well as quantify virtually all fluorescent dye from visible to NIR using VisionWorks®LS acquisition and analysis software. The use of NIR and visible fluorescent labels permit multiplexing so that several proteins in a sample can be detected and analysed at the same time on a single protein blot. UVP offers several filters used for NIR blotting with 680 and 770 to 800nm fluorescent tags.

Routine NIR imaging with the BioSpectrum and BioLite MultiSpectral Source is fast, efficient and straightforward yielding full 16-bit images for quantitation and publication.