Deep in Vivo Imaging with New Multiphoton Excitation (mpe) Microscope Range

Olympus, has introduced a Multiphoton* deep fluorescent imaging system. Based on the unique Olympus FluoView FV1000 confocal Laser Scanning Microscope (cLSM), the MPE system is available in three models providing the highest penetration depths on the market. Each model incorporates Olympus's market leading components, such as the UIS2 optics including among others well-known long working distance water-immersion objectives. The systems are fully configured to ensure best excitation efficiency and thus imaging hundreds of microns deep into a specimen becomes possible.

Each of the three models in the Olympus FV1000MPE range uses IR femtosecond pulsed lasers and non-descanned detectors to cover every multiphoton excitation imaging requirement. The entry level model is designed to achieve maximum penetration depth with minimal complexity. The advanced models offer IR laser attenuation via an acousto-optical modulator (AOM). This enables the use of all the sophisticated scanning and laser control modes of the FV1000, such as ?region of interest' imaging and fly back beam blanking. The carefully designed laser introduction path with AOM laser control provides negative-chirp laser beam conditioning for the optimum fluorochrome excitation efficiency in MPE applications. This produces a pinpoint photon density precisely at the focal plane in the specimen with minimal damage or photobleaching to living cells.

The third model incorporates the unique SIM scanner, available for the Olympus FluoView FV1000. This system provides an independantly controllable, fully integrated second IR laser for simultaneous multiphoton imaging and multiphoton laser manipulation. A solution, which is essential e.g. in uncaging applications deep within the tissue. This provides a unique system for the ultimate in multiphoton microscopy.