dsDNA System for Sensitive Add-and-Read DNA Quantitation Introduced

Promega Corporation (USA) announced today the launch of the QuantiFluor ONE dsDNA System, a sensitive, dsDNA-specific fluorescent dye suitable for use in both research and clinical workflows. The new dye system is integrated into the new Promega Quantus Fluorometer and GloMax detection instruments, but is compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra.

The QuantiFluor ONE dsDNA System is supplied in ‘add-and-read’ format, enabling users to simply quantitate even low concentration samples with confidence, and without dilutions or additional tubes. The dye system has significantly increased sensitivity compared to absorbance based systems and is highly specific to dsDNA, showing minimal binding to ssDNA, RNA, protein and interfering compounds.

The QuantiFluor ONE dsDNA System expands the company’s range of nucleic acid quantitation reagents and instrumentation, which includes the recently launched Quantus Fluorometer for highly sensitive (up to 40,000x higher than absorbance-based methods) quantitation of dsDNA, ssDNA, and RNA. Quantus’ increased sensitivity and range leads to higher success rates in downstream applications such as PCR cloning, transfection and next-generation sequencing.  Quantus is also the ideal solution when working with samples such as FFPE tissue which contain low levels of nucleic acid, enabling users to move on to downstream detection assays knowing that DNA or RNA is present.

Other dyes for use with Quantus include dsDNA System, ssDNA System, and RNA System. Quantus is optimised for use with QuantiFluor Dyes, but is also compatible with other fluorescent quantitation dyes.