HTRF® - Certification for the Multimode Microplate Reader

Berthold Technologies is ready to announce the HTRF®-certification for the multimode microplate reader Mithras LB 940. The HTRF® assay platform
from Cisbio offers the researchers a highly sensitive, robust technology for the detection of molecular interactions of proteins in vitro and widely used by the pharmaceutical and biotech industry for the high throughput screening stage of drug development. With its HTRF® technology, Cisbio offers a comprehensive technological platform for the screening and investigation of biological interactions such as G-Protein Coupled Receptor (GPCR), kinase and imflammation biomarkers among others. Berthold Technologies has based its development on DOPS – Dedicated Optical Path System - to ensure the best available performance for each technology.
Mithras LB 940 offers all comprehensive technologies used in modern research including: absorbance; luminescence; BRET; AlphaScreen®;
fluorescence; FRET; fluorescence Polarisation (FP); time-resolved fluorescence; and HTRF®. Mithras can be supplemented with up to 4 reagent injectors – based on the proven and highly precise and accurate JET injection technology – and a temperature control unit for the microplates. All these features offer extensive opportunities to the users to perform a huge number of applications including kinase activities, second messengers (e.g. cAMP, IPOne), GPCRs, phagocytosis, calcium flux, cell viability, apoptosis, protein and DNA determinations, reporter gene assays or protein-protein interactions.
The Time-Resolved Fluorescence module uses a high-power flash lamp. Optical emission filters can be used in Mithras´s high-efficiency luminescence optics (BRET (e.g. functional assays for GPCR research) and multicolour luminescence (luciferase reporter gene assays). Alphascreen™ can also be measured on the Mithras multimode reader. The software is supporting
a multitude of functions including standard curves and kinetic data reduction and meets the 21 CFR part 11 requirements. For higher throughput Mithras LB 940 may be equipped with the Stacker unit LB
931 or the BUTLER LB 930 plate handler or it can be integrated into a laboratory automation system.