Multi-Parameter Flow Cytometer Delivers High Complexity Cellular Analysis

Beckman Coulter Life Sciences continues to expand the multi-parameter capabilities of its patented CytoFLEX flow cytometer technology. Its latest model, the CytoFLEX LX, with up to six lasers and 21 fluorescent channels, delivers even more parameters with the same exceptional fluorescence sensitivity and superior nanoparticle detection demanded by high end researchers.

With the CytoFLEX LX series, the platform’s innovative excitation and detection capabilities have been further leveraged to meet the high-content needs of researchers interested in up to 23 parameters of cellular analytical measurements.

The ease of use and maintenance of CytoFLEX LX now enables many more researchers to use the system and push their science to this high level. Moreover, other labs which typically used shared lab resources for such complex experiments can now consider accelerating their work with their own benchtop system.

The CytoFLEX sets new standards of fluorescence sensitivity by minimising light loss, so that even dim populations can easily be detected. It can measure as low as ten molecules of fluorescence and measure particles as small as 0.2 micrometers in size.

With the CytoFLEX LX, researchers can measure two scatter parameters in addition to up to 19 fluorescent markers with five lasers; and up to 21 fluorescent markers when six lasers are activated. The patent-pending optical design of the system optimises excitation and light collection efficiency from up to six lasers (375, 405, 488, 561, 638, and 808 nm).

A Wavelength Division Multiplexing (WDM) detection module (also patent-pending) features a bandpass-only design that enables various configurations from four to 21 colours, and contains the full complement of repositionable optical filters.

A new version of Beckman Coulter’s CytoFLEX acquisition software, CytExpert Version 2.0, has also been introduced and is available on the new CytoFLEX LX series. By means of a novel compensation library, users are easily able to set up high colour applications, whether they are in the lab or working remotely.

With CytExpert Version 2.0, researchers can compare and reproduce experimental data over time and across multiple systems, confident that they are reporting accurate and standardised results for each specific application. Other features ideal for high throughput labs include microtitre plate-based analytics; as well as the ability to interface with front-end automation systems. The software also provides tools facilitating compliance with 21CFR11 regulations such as user management, audit trails, and electronic signatures.