Extending Confocal and Multiphoton Functionality

Olympus has introduced advanced laser sharing and dual port SIM scanning capabilities to its FluoView FV1000 confocal laser scanning and FluoView FV1000MPE multiphoton laser scanning microscopes. Providing effective and efficient methods of increasing flexibility, these modules enable users to perform a diverse range of applications, without the need to invest in additional imaging systems.

The innovative Olympus laser sharing dual microscope system enables users to utilise the same IR laser on two MPE systems. As a result, researchers can carry out a diverse range of applications, linking multiple multiphoton systems based on inverted and/or upright microscopes.

The addition of a second laser port in the Olympus FV1000 SIM scanner increases the versatility of the Olympus confocal and multiphoton systems. For example, it becomes possible for researchers to use IR and visible light simultaneously for stimulation experiments. In addition, both lasers can be used alternately for ultra-fast multi-point scanning applications.

The Olympus FluoView FV1000MPE range provides excellent deep imaging for live and thick slice samples. Based on the unique Olympus FluoView FV1000 confocal Laser Scanning Microscope (cLSM), the MPE system is available in four models, providing the highest penetration depths on the market. Each model uses prechirped IR femtosecond pulsed lasers and non-descanned detectors to cover every mutiphoton excitation image required. The ‘Multiphoton exclusive system’ is designed to achieve deeper imaging with minimum complexity, whereas the ‘Basic system’ enables multiphoton deep imaging and confocal imaging with a visible laser. The ‘Stimulation system’ is equipped with two sets of laser scanners for confocal and simultaneous stimulation using multiphoton absorption. As the most advanced model, the ‘Twin system’ incorporates the unique SIM scanner, providing an independently controllable, fully integrated second IR laser for simultaneous multiphoton imaging and laser manipulation