Antibody Labelling Guide

Innova Biosciences has launched a new antibody labelling guide to provide greater awareness of how easy it is for scientists to label primary antibodies within their own laboratories.

The free publication discusses the differences between direct and indirect immuno detection, various approaches to antibody labelling, and buffer formulations and antibody concentration.

Also discussed is Innova Bioscience’s Lightning Link, a simple and quick method for antibody labeling. The antibody labelling guide is also quick and concise; consisting of 9 informative pages.

Direct labelling of antibodies greatly simplifies immunodetection techniques. Without the problems of crossover and/or nonspecific binding from secondary reagents it is far easier to obtain high quality results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry.

A research group that has benefited from use of the Lightning-Link range is headed by Graham Pockley, the Professor of Immunobiology at the University of Sheffield, who commented: “My research depends heavily on multi-colour flow cytometry and it was becoming increasingly difficult to commercially source the appropriate labelled antibodies for our studies.

“When I discovered the Lightning-Link technology I was really overwhelmed about the simplicity of the kits. More importantly, they were perfectly suited to our needs, as their ‘no loss’ labelling strategy means that we can purchase small aliquots of unlabeled antibody / isotype controls and label these in house. The ability to do this has transformed our work and I would highly recommend the kits to anyone in the research community.”

Innova is a pioneer in the field of antibody labelling (conjugation). Lightning-Link™ simplifies the development and production of conjugates for immunodiagnostic reagents. Irrespective of scale, the hands-on time for setting up a labelling reaction is less than 30 seconds. Recovery of antibody approaches 100%.

Batch-to-batch variation is all but eliminated. Despite its simplicity, Lightning-Link™ is a very sophisticated conjugation system in which the antibody is directionally coupled to the label in a controlled fashion at neutral pH, creating high quality stable conjugates. All popular labels are available in a one-step kit format.