Improved Cryopreservation of Human Hepatocytes

An article from researchers at the Karolinska Institute details an improved protocol for cryopreservation of primary human hepatocyte using STEM-CELLBANKER^® cryopreservation solution from Amsbio.

In the article the authors compare cryopreservation of liver hepatocytes using STEM-CELLBANKER with the frequently used DMSO-University of Wisconsin (DMSO-UW) medium protocol. Both protocols yielded hepatocytes with good P450 function, however, the STEM-CELLBANKER protocol gave a higher viability than the DMSO-UW cryoprotectant. The researchers concluded that STEM-CELLBANKER beneficially is also xeno-free and will be applicable to cryopreservation of clinical-grade primary human hepatocytes.

STEM-CELLBANKER from Amsbio is a chemically defined freezing medium with optimised formulation for stem cells and iPS cells storage as well as other valuable cells. Supplied ready-to-use with a simple usage protocol, STEM-CELLBANKER is completely free of serum and animal derived components, and contains only European or US Pharmacopoeia graded ingredients. Part of the CELLBANKER® series of cell freezing media, STEM-CELLBANKER^® significantly increases cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability following resuscitation from cryopreservation, even after extended long-term storage. Every batch of STEM-CELLBANKER® cryopreservation medium is performance tested on Jurkat and SK-007 cells. Additional standard evaluations for endotoxins, pH, osmolarity and Mycoplasma contaminants are undertaken to ensure GMP equivalent quality.