COMPUTER-ASSISTED OPTIMISATION METHOD FOR MODELLING AND PREDICTION THE RETENTION BEHAVIOUR OF ACIDIC COMPOUNDS ON A FLUORINATED PACKING

Part 1 of this article appeared in the January 2008 issue of International Labmate and gave the background to the work carried out by the authors alongside experimental detail and a discussion of some of the Results obtained using the methodology of screening significant factors using the orthogonal array (OA)design. In Part 2 the article discussion centres on the use of Optimisations with the aid of RSM and central composite design,specifically Polynomial mathematical models of retention time and resolution and Optimisation of separation with the aid the overlapping contour plot. Conclusions are then drawn on the results obtained and presented.