Experience newfound clarity with the Nexera XS inert UHPLC

Offering reliable, robust performance, the Nexera XS inert represents a new peak in the analysis of biopolymers. The system has been designed to resolve the most common problems encountered in the analysis of biopharmaceuticals, such as the adsorption of target molecules to metal surfaces and corrosion due to mobile phases with high salt content and extreme pH conditions. The development of the Nexera XS inert was based on the results of cooperative research with Professor Yasushi Ishihama of the Graduate School of Pharmaceutical Sciences at Kyoto University.

The new Nexera XS inert UHPLC features a metal-free sample flow path that prevents unwanted interaction with biopolymers prone to adsorption onto exposed metal sites. By doing this, the system achieves reduced sample loss and excellent peak shape for reproducibly high sensitivity and high-quality, reliable data. In addition, all wetted surfaces are prepared from corrosion-resistant materials, rendering them stable against mobile phase solvents containing high concentrations of salts or acids.

The Nexera XS inert offers the same superior reliability, robustness and expandability as the other UHPLC systems in the Nexera series. It is particularly well-suited to the analysis of biopolymers such as antibodies, peptides and nucleic acid drugs.

In addition to the Analytical Intelligence features of the Nexera series, the Nexera XS inert system offers:

• Unconstrained Recovery and Sensitivity

In quantitative analysis of biomolecules, adsorption of the target compound to a metal surface can result in a dramatic decrease in sensitivity. The Nexera XS inert offers a bioinert flow path to prevent adsorption to metal surfaces. It therefore achieves much higher sensitivity and better reproducibility than a general-purpose UHPLC system.

• Assured Reliability and Reproducibility

High salt conditions in the mobile phase can result in corrosion of stainless-steel parts in standard UHPLC systems. The Nexera XS inert system is unaffected by high salt content, ion pairing agents or extreme pH conditions, ensuring long-term stability and reliable data acquisition.

• Clear Resolution without Restrictions

The system’s 105 MPa pressure resistance enables the use of long separation columns packed with small-sized particles, to achieve ultra-high efficiency in a wide variety of applications. The newly developed tool-free, finger-tightened fittings permit reliably connected columns to withstand a pressure of up to 105 MPa without creating any dead volume or risking damage by overtightening. All valves used in the Nexera XS inert system are designed to inhibit metal adsorption, allowing for worry-free switching between multiple columns for method development or for trap-and-elute analysis. The optional pHM-40 offers real-time measurement of the mobile phase pH, to ensure accuracy and traceability, even when running a pH gradient, as used in ion-exchange chromatography.

To learn more about the Nexera XS inert and its features follow this link.