Automated Alternative to Immunofixation Provides Perfect Resolution

Expert in clinical electrophoresis, Sebia offers a range of automated capillary and agarose gel systems for immunology testing. The immunotyping (IT) assay utilises Capillarys’ proven capillary electrophoresis technology to provide an automated alternative to traditional immunofixation testing.

Following detection of paraproteins using routine protein electrophoresis, immunofixation is used to identify monoclonal antibodies or immunoglobulins present. Sebia’s IT assay is a completely automated method
allowing up to 10 samples to be processed per hour. After loading primary sample tubes onto Capillarys, each serum is mixed with specific antisera, such as anti IgG, IgA, IgM, kappa and lambda. An antigen-antibody complex is rapidly formed in the liquid medium, with no external sample mixing or incubation required. Treated samples are then automatically analysed allowing laboratory personnel to walk away after initial sample loading.

A reference pattern is overlaid with each antisera pattern to allow easy identification of the monoclonal peaks, which have been removed. With excellent resolution and method sensitivity of 25-50 mg/ml, the fully-automated immunotyping method from Sebia is a perfect alternative to traditional immunofixation. For laboratories with limited bench space, the IT assay is also available on Sebia’s compact capillary electrophoresis system, Minicap.