Minimum Sample Volume for Biomolecule Characterisation

Malvern’s new Zetasizer μV is an advanced light scattering system specifically intended for the characterisation of proteins and other biomolecules. This latest addition to Malvern’s highly successful Zetasizer family is designed and optimised for maximum sensitivity and minimum sample volume. The Zetasizer μV is a dedicated system that supports improved understanding and management of a wide range of biomolecule applications, including: optimising sample and storage conditions; verifying the quaternary structure of protein complexes; identifying thermal characteristics; and assessing protein solubility and sample purity. The amount of protein available, particularly during early stages of discovery is often very limited, so the ability to measure very small quantities is critical. The Zetasizer μV requires just two microlitres of sample, allowing measurement of as little as 40 picograms of BSA (bovine serum albumin). Sample is recoverable, ensuring no loss of valuable material. The use of SOP’s (standard Operating procedures) means that the measurements proceed with no user input and are managed and supported by the proven software used throughout the Zetasizer range.

The Zetasizer μV employs both dynamic (DLS) and static light scattering (SLS). DLS determines the diffusion speed of proteins and nanoparticles in solution to give accurate measurement of size and size distribution. SLS enables determination of absolute molecular weight and 2nd virial coefficient, a parameter used to assess protein solubility and suitable conditions
for crystallisation.

However, the real value of the Zetasizer μV lies in using the measured properties to help solve application problems in bioprocessing, drug target development and protein therapeutics.