White Wells Can Improve qPCR Results

Every researcher doing real-time PCR always wonders about the best instrument, the best mastermix, the best polymerase and so on. However, when it comes to the plastic consumables that build the connection between instruments and reagents, one does not expect any influence on the final results. Eppendorf can demonstrate significant improvement of real-time PCR data using a new PCR plate. With this new twin.tec real-time PCR plate, Titanium dioxide gives the reaction chambers a bright
white, opaque colour. This leads up to a 10-fold increase in reflection of fluorescence, which provides better data for cutting-edge applications such as low volume qPCR. Due to the more homogenous background of the white colour compared to clear wells, data from replicates becomes more homogenous. This results in lower standard deviation over a plate, which can be crucial to further process the data in multivariate analyses.