New High Fidelity DNA Polymerase

Thermo Fisher Scientific, Inc announced the new Thermo Scientific Verbatim High Fidelity DNA Polymerase. This unique, advanced DNA polymerase allows highly accurate amplification of DNA templates, equivalent to industry leading proofreading enzymes, while maintaining processing speed and delivering high yields. These high-performance characteristics make Verbatim High Fidelity DNA Polymerase ideal for demanding downstream applications such as cloning, site-directed mutagenesis and sequencing. High Fidelity PCR requires a DNA polymerase with proofreading capabilities, and should be used in applications requiring accurate amplification of a DNA target. Thermo Scientific demonstrated the advanced proofreading capabilities of Verbatim High Fidelity DNA Polymerase during in an in-house comparative evaluation. Verbatim demonstrated its industry leading performance by delivering greater enzyme fidelity than all other enzymes tested1 (Taq DNA polymerase, Pfu DNA polymerase/Taq blend, KOD DNA polymerase and a leading fusion enzyme).

Verbatim High Fidelity DNA Polymerase also demonstrates increased affinity for DNA templates, which significantly improves yield and processivity. This increased processivity allows PCR protocol times to be reduced by up to 75 percent, for faster results. Enhanced DNA binding affinity also ensures that Verbatim is suitable for a broad range of templates, and the enzyme is supplied with two optimised buffering systems - one for standard templates and the other for more complex targets (such as GC-rich templates). Thermo Scientific Verbatim High Fidelity DNA Polymerase kits (with sufficient reagents for 200 or 1000 reactions) are available with or without dNTPs.