HALO BioClass for fast, high resolution separations of biomolecules

HALO BioClass columns are specifically designed for fast, high-resolution separations of proteins, peptides and other biomolecules and are especially suited to work involving:

• Intact proteins, monoclonal antibodies (mAbs), biosimilars and other large biomolecules such as pegylated proteins and antibody drug conjugates (ADCs);
• Peptide mapping (analysis of enzyme digest) for characterisation and monitoring of synthetic protein drugs;
• Analysis of therapeutic peptides and peptide biomarkers (protein surrogates); and
• High resolution separations of complex mixtures of glycans released from N- and O-linked glycoproteins.

The range currently includes three product types and five chemistries:

1. HALO Protein columns are specifically designed for the efficient separation of proteins. They are available in two chemistries, HALO Protein C4 and HALO Protein ES-C18. Both are stable up to 90°C and can elute very large proteins with good peek shape and recovery. They offer low LC-MS bleed and are compatible with HPLC and UHPLC.
2. HALO Peptide columns are designed for fast high resolution separation of peptides. They are available in two chemistries, HALO Peptide ES-C18 and HALO Peptide ES-CN. They are extremely stable up to 90°C and offer rugged and reliable performance. Both can be used with HPLC and UHPLC.
3. HALO Glycan columns offer improved retention of acidic and zwitterionic analytes with very low sensitivity to buffer concentration. The unique phase incorporates a highly polar ligand that contains five hydroxyl groups tethered to a 2.7um Fused-Core silica particle via novel proprietary chemistry. It is ideal for the HILIC separation of oligosaccharides, and particularly, of released and labelled glycans from glycoproteins and proteoglycans.

For further information on any HALO BioClass products please click here or contact Hichrom Limited on +44 (0) 1189 303660 or email.