New assay kit simplifies mitochondrial function analysis

Agilent Technologies has introduced the Mito-rOCR Assay Kit, a streamlined solution that simplifies the analysis of mitochondrial function for researchers of all skill levels. This cost-effective kit enables functional assessment of mitochondrial respiration in cell physiology and disease pathology studies, making advanced mitochondrial research more accessible.

Mitochondria play a crucial role in energy production, and mitochondrial dysfunction is linked to various diseases. The Mito-rOCR Assay Kit provides an easy and reliable way to measure relative oxygen consumption rate (rOCR), offering insights into mitochondrial health. Researchers can use the assay with a range of compatible fluorescence plate readers and multimode imagers that meet sensitivity, detection, and environmental control requirements.

The BioTek Cytation series, ideal for use with the Mito-rOCR assay, supports automated processes and ensures optimal conditions for diverse research needs. The assay measures rOCR, enabling sensitive detection of changes in mitochondrial function without interfering with cellular processes. The reagent remains outside the cells, ensuring it can be easily removed for further downstream analysis.

Data analysis is simplified using the Mito-rOCR Analysis Module within Agilent Seahorse Analytics, a cloud-based software that makes data import, linearisation, and chart creation easy and efficient.

Chris Braun, Associate Vice President of Marketing in Agilent’s Cell Analysis Division, commented: “The Mito-rOCR Assay Kit was developed in response to customer feedback, providing an easy-to-use assay that works with various fluorescence plate readers. This kit empowers more researchers to study cellular respiration and mitochondrial function.”

The Mito-rOCR Assay Kit is part of Agilent’s expanding suite of metabolic analysis tools, offering researchers a comprehensive view of cellular metabolism and supporting advancements in biological research.

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