Chromatography
Untargeted Metabolomics Using Orbitrap-Based GC-MS
Jul 07 2015
Application note 10457 demonstrates the application of a complete untargeted metabolomics workflow using a novel Thermo Scientific™ Orbitrap™ MS-based GC to detect biomarkers for time of death in a rat model. Estimation of post-mortem interval (PMI) is one of the most critical, yet difficult, tasks in forensic investigation, particularly after the cadaver has equilibrated to the ambient environmental
temperature. Current methods to determine PMI are inaccurate and primarily based on visual inspection of the body. A laboratory-based method, using a robust biomarker for PMI, would assist forensic investigation.
This GC-MS configuration using an Orbitrap-based detector enables ultra-high mass resolution, sub-ppm mass accuracy, a large dynamic range, and a scan rate commensurate with the efficient quantitative analysis of highly complex metabolomic samples. The high resolution, mass accuracy, and scan speed is critical for consistent data deconvolution to permit the detection of species from overlapping TIC peaks, allowing for an untargeted metabolomics pipeline. Accurate mass electron
ionisation (EI) fragment patterns are also suitable for matching against the widely available NIST and Wiley libraries for tentative compound identification, while providing accurate mass for more in-depth characterisation.
Read more on http://ilmt.co/PL/QYjz
Digital Edition
International Labmate Buyers' Guide 2024/25
June 2024
Buyers' Guide featuring: Product Listings & Manufacturers Directory Chromatography Articles - Enhancing HPLC Field Service with fast-response, non-invasive flowmeters - Digital transformatio...
View all digital editions
Events
Jul 03 2024 Gandhinagar, India
Jul 07 2024 Dublin, Ireland
Jul 20 2024 Denver, CO, USA
Jul 21 2024 Cape Town, South Africa
Jul 28 2024 San Diego, CA USA