Chromatography
Probes of Molecular Chirality & Structure
Jun 13 2008
Polarisation of fluorescence (POF) and Circularly Polarised Luminescence (CPL) are less utilised highly sensitive probes of molecular interactions (e.g. protein folding, secondary structure formation).
Whereas CD involves the ground state of a chiral molecule, CPL is emitted by a fluorophore if the excited state of the emitting species is chiral. Fluorescence offers insights into the environment surrounding the emitting molecule; anisotropy (POF) is sensitive to rotational motion of the fluorescent probe.
These four probes provide complementary information, with POF and CPL providing insights into the structure, interactions, and environment of the emitting molecule.
A recently introduced Olis upgrade accessory adds both CPL and POF to any Olis CD or fluorescence spectrometer for kinetic or steady-state readings. Single purpose models are also available.
Digital Edition
International Labmate Buyers' Guide 2024/25
June 2024
Buyers' Guide featuring: Product Listings & Manufacturers Directory Chromatography Articles - Enhancing HPLC Field Service with fast-response, non-invasive flowmeters - Digital transformatio...
View all digital editions
Events
Jul 03 2024 Gandhinagar, India
Jul 07 2024 Dublin, Ireland
Jul 20 2024 Denver, CO, USA
Jul 21 2024 Cape Town, South Africa
Jul 28 2024 San Diego, CA USA