Chromatography
New ?chromatography Flow-mode? Option Transforms Zetasizer Nano Into In-line Detector for Absolute Size Exclusion Chromatography
May 02 2007
A significant new development, ?chromatography flow-mode' permits the coupling of dynamic light scattering with size exclusion chromatography, conferring the ability to perform Absolute Size Exclusion Chromatography (ASEC). This allows protein size to be measured directly rather than relying on column calibration or additional data from a second detector, and means that molecular weight standards are no longer required to identify oligomeric species or determine protein purity.
To use the Zetasizer Nano as an absolute light scattering detector it simply needs to be connected after the last detector in a size exclusion chromatography (SEC) system. The instrument can be added to any SEC set-up; no column calibration is required and the Zetasizer Nano's software makes the entire measurement process simple and straightforward. For the majority of protein separations in aqueous media, no extra parameters are required. Size is plotted in real time as material is eluted from the column, and the molecular weight of each peak is calculated automatically at the end of the measurement.
The Zetasizer Nano was designed specifically to meet the low concentration and small sample volume requirements of protein and other biomolecular applications and is now in widespread use. It is by applying the system's patented optical design and high sensitivity capabilities to real time flow measurements that Malvern has facilitated the Zetasizer Nano's use as a chromatography detector, providing a major new tool for protein researchers.
Digital Edition
Lab Asia 31.3 June 2024
June 2024
Chromatography Articles - Enhancing HPLC Field Service with fast-response, non-invasive flowmeters Mass Spectrometry & Spectroscopy Articles - UV-Vis and fluorescence spectroscopy illumina...
View all digital editions
Events
Jun 26 2024 Tokyo, Japan
Jul 03 2024 Gandhinagar, India
Jul 07 2024 Dublin, Ireland
Jul 20 2024 Denver, CO, USA
Jul 21 2024 Cape Town, South Africa