Chromatography
Identification and confirmation of substances in research and routine analysis by hyphenation of HPTLC with MS
Oct 07 2015
CAMAG, a leading manufacturer of HPTLC equipment, recently announced the worldwide availability of the new CAMAG TLC-MS Interface 2, the second generation of its module for the pioneering concept of hyphenating HPTLC with mass spectrometry. While the visual appearance remains virtually unchanged, the CAMAG TLC-MS Interface 2 features a modified elution head and an easily accessible, exchangeable filter, arranged in front of the valve. Cleaning is facilitated as compared to the previous version, making it highly efficient. By pushing a button, the elution path is cleaned of matrix particles with compressed air, increasing the lifetime of the filter and preventing the system from becoming blocked. These filters can be easily replaced without any modification to the elution head.
With the new CAMAG TLC-MS Interface 2, plate positioning is significantly simplified: together with the positioning scale and the adjustable plate-stopper, the integrated crosshair laser enables accurate and reproducible positioning of the plate. Alternatively the coordinates determined by the CAMAG TLC Visualizer or the CAMAG TLC Scanner can be used to precisely position the HPTLC plate.
The CAMAG TLC-MS Interface 2 can be installed plug & play with any LC-MS system without adjustments or mass spectrometer modifications. Depending on the MS system, a substance can be identified within a minute via its mass spectrum, or for an unknown substance zone, the respective sum formula can be obtained. Furthermore, interesting zones can be eluted into vials for further investigations with, e.g, NMR, (ATR-)FTIR, ESI-MS, and MALDI-MS.
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