SIELC – BIST™ Technology

Best separation science innovation

Recently discovered SIELC Technologies’ Bridge Ion Separation Technology (BIST™) HPLC separation mode deserves to win the Labmate Award for Excellence because it represents a groundbreaking advancement in HPLC separations, offering a unique and orthogonal solution for retaining and separating charged molecules.

Have you been looking for a new, efficient way to retain and separate charged molecules in your HPLC application? Have you had problems retaining or eluting polar ionic molecules with multiple charges?

With the revolutionary new Bridge Ion Separation Technology (BIST™), you can put those concerns to rest! SIELC Technologies’ BIST™ is a novel and innovative approach that enables separations previously considered difficult or even impossible with classical separation modes.

What Makes BIST™ a Game-Changer?
BIST™ Mechanism and Properties:
Utilizes double-charged ions in the mobile phase to create a bridge-type interaction between the stationary phase surface and analytes of the same charge, enabling improved selectivity and retention control. BIST™ is a unique separation mode where the charge of analytes and the charge of the column surface are the same.
In contrast to ion-exclusion mode, where separation occurs in pre-void time, the BIST™ separation occurs in retention time, so it is not limited.
Allows convenient control of the degree of retention of charged molecules just by changing the water content in the mobile phase, offering unprecedented control over retention.
Unlike traditional Ion-Exchange, HILIC, or Reversed-Phase techniques, BIST™ provides a distinct separation mechanism that is minimally affected by ionic strength but is sensitive to water concentration.
Why BIST™ is Revolutionary
Traditional Ion Exchange Chromatography (IEC) relies on electrostatic attraction between the analyte and the stationary phase. However, BIST™ introduces an entirely new way to manipulate interactions by leveraging double-charged ions to alter the charge characteristics of the stationary phase. This breakthrough provides retention for analytes that would otherwise elute in the void.

Real-World Impact
BIST™ allows scientists to efficiently retain and separate single- and multi-charged ionic compounds, particularly those challenging to analyze using conventional techniques, significantly reducing method development time and improving accuracy.

Proven Excellence
U.S. Patents Pending.
Several peer-reviewed publications have been issued.
Multiple real-life applications have been developed with the BIST™ approach.
With its ability to redefine the way charged molecules are retained and separated, BIST™ is an innovation worthy of the Labmate Award for Excellence."