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Maximising Core-Shell Performance on Conventional HPLC Systems

Jul 09 2012

Author: Jeff Layne and Simon Lomas on behalf of Phenomenex Inc

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In order to fully realise the performance potential of core-shell columns on conventional HPLC systems, some simple system optimisations maybe necessary to minimise extra-column band broadening effects. In this report, a series of simple measures (adjusting detector acquisition rate; optimising connective tubing; use of a low-volume flow cell) are made to a standard HPLC system in order to achieve UHPLC-comparable performance with sub-3μm core-shell media.

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Chromatography

Maximising Core-Shell Performance on Conventional HPLC Systems

Author: Jeff Layne and Simon Lomas on behalf of Phenomenex Inc

Free to read

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Free to read

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