Chromatography
Increased Sensitivity, Improved Resolution and Faster Analysis Times For Compendial Methods
Dec 23 2009
Author: Elli Abbasi, Jeff Layne, Heiko Behr, and Sky Countryman on behalf of Phenomenex Inc
Determination of Impurities and Related Substances Using Newly Developed 2.6 μm Core-Shell Kinetex™ LC Columns
Presently, HPLC methods for the determination of impurities and related substances of drug products specified in monographs by the various Pharmacopoeial agencies typically employ LC columns packed with fully-porous 3 or 5 micron spherical silica chromatographic media. Due to the performance limitations of fully-porous 3 and 5 micron spherical silica chromatographic media, these analytical methods commonly require long analysis times.
Additionally, accurate quantitation of low-level impurities in routine LC-UV applications may be challenging due to the low intensity peaks generated by these columns. In recent years, smaller fully-porous LC particles
(sub-2 micron diameter) have been introduced that offer faster analysis times and generate higher intensity peaks for better sensitivity. Unfortunately, widespread adoption of this sub-2 micron HPLC column technology has been slowed since the smaller particle columns generate system backpressures that require specialised ultra-high pressure capable LC instrumentation.
Digital Edition
LMUK 49.7 Nov 2024
November 2024
Articles - They’re burning the labs... Spotlight Features - Incubators, Freezers & Cooling Equipment - Pumps, Valves & Liquid Handling - Clinical, Medical & Diagnostic Products News...
View all digital editions
Events
Nov 11 2024 Dusseldorf, Germany
Nov 12 2024 Cologne, Germany
Nov 12 2024 Tel Aviv, Israel
Nov 18 2024 Shanghai, China
Nov 20 2024 Karachi, Pakistan