New System for Effortless Ultra High Pressure LC-MS Proteomics Analysis

Thermo Fisher Scientific, Inc announced the launch of a new split-free, nano-flow UHPLC system. The Thermo Scientific EASY-nLC 1000 is the newest addition to the EASY-nLC family of nano-LC products. The EASY-nLC 1000 is unique in its ability to facilitate dedicated separation of biomolecules, such as proteins and peptides, at ultra-high pressures. The instrument is extremely easy to use and can seamlessly integrate with the complete range of Thermo Scientific mass spectrometry (MS) systems for instant, trouble-free operation. The Thermo Scientific EASY-nLC 1000 uses shorter gradients on 50μm UHPLC columns with 1.9μm beads, delivering superior separation capability with razor-sharp peaks. As a result of its advanced design, the EASY-nLC 1000 facilitates as much as 30% more
peptide and protein identifications by providing higher pressure ratings, along with independent and simultaneous variations of bead size and column length. Increasing the number of identifications is a significant achievement for proteomic research and discovery purposes. The EASY-nLC 1000 operates on the Thermo Scientific Xcalibur software platform, easily connecting and interfacing with the full suite of Thermo Scientific LC-MS systems.

The higher operating pressure range of the EASY-nLC 1000 significantly shortens analytical cycles and is designed to increase productivity by accelerating sample loading and column reequilibrations. Additionally, the split-free, nano-flow capability of the new system provides excellent retention-time reproducibility (below 0.4% RSD), even for very complex samples. The simple integrated configuration is both easy-to-use and robust. “We believe the EASY-nLC 1000 will be a market-leading instrument because it offers UHPLC performance that is dedicated to proteomics scientists,” said Andrew Altman, Vice President and General Manager of liquid chromatography, Thermo Fisher Scientific. “This capability is complemented by exceptional ease of use, rapid operation and better separations in a highly robust instrument. The EASY-nLC 1000 will revolutionise the way LC-MS analyses are performed.”