Chromatography
Protein Standards for Mass Spectrometry Analysis
Sep 23 2010
Amsbio has announced a new service whereby it can generate high quality Mass Spectrometry (MS) standards for over 5,000 human proteins. Produced in human HEK293T cells and labelled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-LLysine, the full-length proteins with appropriate post-translational modifications are the best choice for accurate identification and quantification of protein biomarkers.
Amsbio offers stable isotope-labelled full-length protein standards for both matrixassisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) Mass Spectrometers enabling researchers to identify the best SRM and MRM transitions through experimental data.
The MS standards commercialised by Amsbio allow researchers to spike at an early stage of sample processing enabling accurate quantification. Produced in human HEK293T cell line the protein MS standards have authentic post-translational modifications with greater than 90% stable isotope labelling incorporation efficiency. As a consequence researchers are able to gain higher data consistency than by using synthetic peptide internal standards.
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