What is the best way to purify plasmid DNA?

What is Plasmid DNA?

Important tools in the life science and biotechnology industry and academic laboratories, plasmid DNA, or simply plasmids, are used to clone and amplify (make many copies of) or express particular genes. A wide variety of plasmids are commercially available for such uses. Plasmids are commonly being used in drug discovery and development for biopharmaceuticals

What are the most commonly used applications of Plasmid DNA?

•    Bacterial vector cloning 
•    Protein production
•    Gene therapy 
•    Different disease models

Can plasmid DNA purification be automated?

The Biotage® PhyPrep from Biotage is an automated platform for plasmid DNA purification. The system releases laboratory staff from repetitive, manual labour and takes full advantage of the PhyTip® columns’ dual flow chromatography technology, to yield supercoiled, endotoxin-free, transfection-grade plasmid DNA.

As each sample is handled in the same way, the new automated Biotage® PhyPrep platform ensures the plasmid yield and purities are more consistent and reduces the risk of human error. Biopharma laboratories outsourcing plasmid purifications now have the option to keep the work in-house, improving process control, project deliverability while at the same time reducing costs.

Biotage® PhyPrep is the first automated solution for plasmid purification in Maxi, Mega and Giga scales. The various scales differ in the volume of cell culture to be purified, with desired yields ranging from 1 mg to 10 mg of plasmid DNA.

“The launch of the Biotage® PhyPrep will assist laboratories in discovering new therapies for patients, utilising biological drug molecules such as antibodies and novel gene therapies. A perfect match with the Biotage ethos of ‘HumanKind Unlimited’ - sustainably releasing the potential of science to address the issues faced by global society, making the world healthier, greener and cleaner,” stated Tomas Blomquist, CEO Biotage.

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