Autosamplers
Protein Applications for Advanced Multi-Detector Size-Exclusion Chromatography
Sep 18 2012
Author: by Mark Pothecary, Stephen Ball, Paul Clarke on behalf of Malvern Instruments Ltd
Size-exclusion chromatography (SEC) or gel permeation chromatography (GPC) is frequently used in bioscience laboratories to characterise purified and recombinant proteins. This technique has also been used to measure molecular weight or to resolve mixtures of monomers, oligomers and higher order aggregates. Using this method, molecular weight is calculated by creating a calibration curve of standard globular proteins and comparing the elution time of an unknown to the standards. This is limited in that it is based on a stable relationship between molecular weight, size, shape or hydrodynamic volume. This relationship varies significantly between proteins introducing unquantifiable inaccuracies in all measurements made in this way.
Events
Mar 18 2025 Beijing, China
Mar 25 2025 Paris, France
Mar 31 2025 Beijing, China
Apr 02 2025 Saigon, Vietnam
Apr 22 2025 Kintex, South Korea