Ultra Fast Preparative Liquid Chromatography
Ultra Fast Preparative Liquid Chromatography
Beyond High-Purity Purification Nexera UFPLC
When developing new drugs, structural analysis of impurities and metabolites is a critical process that cannot be avoided.
However, structural analysis after synthesis or after purification by preparative LC is time consuming and recovering high-purity samples is very difficult. Therefore, as a solution to this problem, Shimadzu offered the Crude2Pure system, which recovers target compounds in high-purity powder based on a revolutionary purification method that involves using two purification systems.
The Ultrahigh-speed Preparative Purification System, Nexera UFPLC system, the newest addition to Shimadzu's family of purification systems, inherits the same purification method as the Crude2Pure, but handles all steps, from fractionation to purification, within a single system, so that target compounds can be recovered in high-purity solution.
Significantly Reduces the Processes Involved from Preparative Work to Purification (Free basing Treatment) and Powderization
With conventional preparative methods, the fractionated sample contains mobile phase solvent, which leads to unavoidable dilution during fractionation, so the fractionated sample must be enriched and desalted. Such procedures complicate the workflow, and amplify sample loss, labor, and costs.
With the Shimadzu UFPLC Ultra Fast Preparative and Purification Liquid Chromatograph, all the work from preparation to enrichment, purification, and recovery is performed on-line. This significantly reduces the labor involved in the preparative purification process, and avoids procedural mistakes. Using a proprietary trap enrichment purification technology for the fractionated target components, trace quantities of components contained in mixtures can be recovered at high concentrations and high purity levels. Additionally, since highly volatile organic solvents are used for the recovery of target components, the time needed for evaporation to dryness can be significantly reduced. When recovering ionic target components, counter ions are flushed out by optimization of the solution flowing through, so the target component is recovered as a high purity free base. With these procedures, and the dedicated Purification Solution™ software, the entire process from the configuration of preparative conditions to the recovery of liquid containing the target compounds can be configured easily from simple and user friendly software.
Building Systems Appropriate to your Purposes
Nexera UFPLC Standard System
This is the most basic Nexera UFPLC system.
Automated Preparative Purification is available when target compound is only one.
Nexera UFPLC Advanced System
This is a fully automated system to purify several compounds, including main target compound, residual substance and impurities (five kinds max.) from preparative sample.
Recover High-Purity Liquid by Eliminating Solvent Components
Easily eliminates previously difficult to eliminate counter ions and low volatility solvents.
Mobile pahses used for HPLC separation for pharmaceutical components sometimes include compounds like trifluoroacetate that forms counter ions. If such components are included in preparative fractions, they can cause problems during drying or testing after purification
However, the Nexera UFPLC system is able to easily eliminate such components that may be contained in fractionated liquids. Depending on the elimination method selected, target compounds can also be recovered in freebase form, in addition to eliminating components from the mobile phase.
Structural Analysis without Pretreatment
Two 0.1% TFA propranolol solutions were prepared, with one treated as a freebase and then the solvent evaporated. The 19F NMR spectra from these two samples are shown. The TFA peaks are much smaller for the sample with freebase treatment than the untreated sample, which indicates that the process was effective in eliminating TFA.
Easy to Operate Dedicated Software
With the dedicated Purification Solution software, the analysis status can be quickly confirmed at a glance using the peak tracking function.
Variety of Fractionation Modes
Automatic Fractionation Mode
In this mode, the software automatically identifies peaks and collects fractions based on parameter settings. Fractions can also be simulated in advance by opening acquired data using the simulation function, which is specialised for optimising parameter settings. It also supports calculating multiple theoretical detector signals.
Manual Fractionation Mode
In this mode, the mouse pointer is used to select peaks for fractionation while viewing the window. If concentrating a sample by reinjecting it multiple times, the function remembers the timing of the first fraction, so that the fraction is automatically performed at the specified time for each injection thereafter.
Time-Specified Fractionation Mode
This mode collects fractions based on the retention times in previously acquired data. It is ideal for routinely performed preparative purification processes.