The PHERAstar® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, Enhanced Dynamic Range technology, UV/vis spectrometer, AAS System, and dedicated AlphaScreen® and TRF lasers, the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.
No longer worry about installing filters and dichroics: the PHERAstar FSX comes with easy-to-handle, assay-optimized Optic Modules which contain all application-specific filters, mirrors, dichroics and/or polarizers, and are automatically recognised by the plate reader. In addition, the PHERAstar FSX is equipped with four matched and assay-optimized photomultiplier tubes (PMTs) which are automatically selected for the relevant detection mode. You can also measure assays with two emission wavelengths at the same time thanks to the Simultaneous Dual Emission.
The Enhanced Dynamic Range and automatic focus technologies make manual settings superfluous and detection optimisation easier than ever.
For HTS automation purposes, the PHERAstar FSX can be easily integrated into automated systems, and offers improved robotic integration capabilities and multi-user control.
Plan to analyse ion channels with a thallium-sensitive dye but don't have an imager? No problem! This adapted assay allows high-throughput detection of ion channel activity on a microplate reader.
Intramolecular bivalent glues can be used for the targeted degradation of proteins. Find out how their binding behaviour can be investigated using microplate readers.
Antibiotic resistance continues to increase. Find out here how BOCILLIN can be used to study resistant penicillin binding protein variants using fluorescence polarization.
The threat of growing antibiotic resistance is constantly increasing. Find out here how venom fractions are emerging as a beacon of hope in the search for novel antibiotics.
Have you heard of dTAG degraders yet? Check out this novel approach in targeted protein degradation and learn how it can be used to identify disease-relevant proteins.
Are you looking for a device to measure sample`s biotoxicity with Aliivibrio fischeri? BMG LABTECH's luminometers fulfil this task and offer additional advantages over the classic cuvette-based analysis.
Mikaeeli, S;Ben Djoudi Ouadda, A;Evagelidis, A;Essalmani, R;Pereira Ramos, O;Fruchart-Gaillard, C;Seidah, N; 2024
Cells
Smith, E;Dickson, L;Pickford, P;Rowland, A;Shumate, J;Perez, K;Scampavia, L;Hernandez, D;Spicer, T; 2024
SLAS Discovery 29(3) 100148
Su, M;Fleisher, T;Grosheva, I;Horev, MB;Olszewska, M;Mattioli, CC;Barr, H;Plotnikov, A;Carvalho, S;Moskovich, Y;Minden, MD;Chapal-Ilani, N;Wainstein, A;Papapetrou, EP;Dezorella, N;Cheng, T;Kaushansky, N;Geiger, B;Shlush, LI; 2024
iScience 27(4) 109443
Wang, R;Yang, Y;Tian, H;Yi, H;Xu, L;Lv, Y;Ge, J;Zhao, Y;Wang, L;Zhou, S;Wang, F; 2024
Genes 15(3)
Graham, K;Lienau, P;Bader, B;Prechtl, S;Naujoks, J;Lesche, R;Weiske, J;Kuehnlenz, J;Brzezinka, K;Potze, L;Zanconato, F;Nicke, B;Montebaur, A;Bone, W;Golfier, S;Kaulfuss, S;Kopitz, C;Pilari, S;Steuber, H;Hayat, S;Kamburov, A;Steffen, A;Schlicker, A;Buchgraber, P;Braeuer, N;Font, NA;Heinrich, T;Kuhnke, L;Nowak-Reppel, K;Stresemann, C;Steigemann, P;Walter, AO;Blotta, S;Ocker, M;Lakner, A;von Nussbaum, F;Mumberg, D;Eis, K;Piccolo, S;Lange, M; 2024
Cell chemical biology
Hu, Z;Chen, PH;Li, W;Krone, M;Zheng, S;Saarbach, J;Velasco, IU;Hines, J;Liu, Y;Crews, CM; 2024
Science advances 10(13) eadj7251
Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning
Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Robot compatible
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Dedicated laser for TRF and TR-FRET
Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer
Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Filters
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
FI
(top)
< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)
Dynamic Range: 8 decades
FI
(bottom)
<1.0 pM FITC (384g black, 50 μL)
FP
< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)
TRF with laser.
< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100% (High Calibrator)
Delta F > 25% (Low Calibrator)
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 8 decades
AlphaScreen® with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD
Flying mode (1 flash)
14 s (384)
27 s (1536)
10 flashes
38 s (384)
1 min 52 s (1536)
50 flashes
1 min 29 s (384)
5 min 18 s (1536)
Linear, orbital, and double-orbital with user-definable time and speed
+5 °C above ambient up to 45 °C
The upper heating plate of the incubation chamber operates at +0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
With Advanced Assay Stability (AAS) system: from 18 - 45°C
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
With AAS system: width: 48 cm, depth: 68 cm, height: 47 cm; weight: 62 kg
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Automatic focal height adjustment (0.1 mm resolution)
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Up to three integrated barcode readers
Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
Microplate incubator and shaker
Magazines for up to 50 plates - continuous loading feature
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
Intelligent microplate incubation
Microplate handling system
Low-volume measurements
Filters for a wide range of applications
Functional Microplate Reader Validation
Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning
Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Robot compatible
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Dedicated laser for TRF and TR-FRET
Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer
Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Filters
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm
FI
(top)
< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)
Dynamic Range: 8 decades
FI
(bottom)
<1.0 pM FITC (384g black, 50 μL)
FP
< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)
TRF with laser.
< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100% (High Calibrator)
Delta F > 25% (Low Calibrator)
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 8 decades
AlphaScreen® with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD
Flying mode (1 flash)
14 s (384)
27 s (1536)
10 flashes
38 s (384)
1 min 52 s (1536)
50 flashes
1 min 29 s (384)
5 min 18 s (1536)
Linear, orbital, and double-orbital with user-definable time and speed
+5 °C above ambient up to 45 °C
The upper heating plate of the incubation chamber operates at +0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
With Advanced Assay Stability (AAS) system: from 18 - 45°C
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
With AAS system: width: 48 cm, depth: 68 cm, height: 47 cm; weight: 62 kg
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Automatic focal height adjustment (0.1 mm resolution)
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
Up to three integrated barcode readers
Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
Microplate incubator and shaker
Magazines for up to 50 plates - continuous loading feature
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
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