The CLARIOstar® Plus is our most flexible multi-mode plate reader. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development.
The CLARIOstar Plus is a multi-mode microplate reader with advanced LVF Monochromators™, highly sensitive filters, and an ultra-fast UV/vis spectrometer. The Enhanced Dynamic Range and automatic focus technologies make manual settings superfluous and detection optimisation easier than ever.
Atmospheric Control Unit, temperature incubation, multiple shaking, well scan and reagent injectors make the CLARIOstar Plus the ideal platform for any live cell-based assay.
The CLARIOstar Plus comes now with Voyager , BMG LABTECH´s new microplate reader control interface. Designed to remove complexity while maximising flexibility, Voyager makes data acquisition more intuitive than ever.
Plan to analyse ion channels with a thallium-sensitive dye but don't have an imager? No problem! This adapted assay allows high-throughput detection of ion channel activity on a microplate reader.
Intramolecular bivalent glues can be used for the targeted degradation of proteins. Find out how their binding behaviour can be investigated using microplate readers.
Antibiotic resistance continues to increase. Find out here how BOCILLIN can be used to study resistant penicillin binding protein variants using fluorescence polarization.
The threat of growing antibiotic resistance is constantly increasing. Find out here how venom fractions are emerging as a beacon of hope in the search for novel antibiotics.
Have you heard of dTAG degraders yet? Check out this novel approach in targeted protein degradation and learn how it can be used to identify disease-relevant proteins.
Looking for a method to study intracellular bacterial cytotoxicity? This AppNote presents a novel method based on the monitoring of propidium iodide uptake with the CLARIOstar Plus.
Yan, Z;Kavanagh, T;da Silva Harrabi, R;Lust, S;Tang, C;Beavil, R;Müller, M;Beavil, A;Ameer‐Beg, S;da Silva, R;Gentleman, E; 2024
Advanced Functional Materials
Yu, V;So, S;Lung, B;Hou, G;Wong, C;Chow, L;Chung, M;Wong, I;Wong, C;Chan, D;Chan, F;Law, B;Xu, K;Tan, Z;Lam, K;Lo, A;Lam, A;Kwong, D;Ko, J;Dai, W;Law, S;Lung, M; 2024
Cancer Letters
Ribeiro, J;Szemerédib, N;Gonçalves, B;Spengler, G;Mateus Afonso, C;Ferreira, M; 2024
RSC Medicinal Chemistry
Nguyen, TTT;Nguyen, TTT;Nguyen, HD;Nguyen, TK;Pham, PTV;Tran, LTT;Pham, HKT;Truong, PCH;Tran, LT;Tran, MH; 2024
Heliyon 10(7) e28118
Bertgen, L;Bökenkamp, JE;Schneckmann, T;Koch, C;Räschle, M;Storchová, Z;Herrmann, JM; 2024
Cell reports 43(4) 114018
Picolo, M;Stephen, A;Baysan, A; 2024
Journal of oral microbiology 16(1) 2327758
Fluorescence intensity (incl. FRET)
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/vis absorbance spectra
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Spectral scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well scanning
6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Robot compatible
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Low-noise photomultiplier tube
Red-shifted photomultiplier tube
CCD spectrometer
Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separates ex & em LVF Monochromators
Optical filters:
Ex and em slides hold 4 filters each
LVF Monochromators + optical filters
Use one for ex and the other for em
UV/Vis absorbance spectrometer:
Full spectra or 8 distinct wavelengths in < 1 sec/well
Filters
FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT)
LUM: 240 - 740 nm
LVF Monochromators™
FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT)
LUM: 320 - 740 nm
Linear Variable Dichroic
340 - 740 nm or 340 - 760 nm (red-shifted PMT)
Spectrometer
ABS: 220 - 1000 nm; wavelength precision: ≤ 0.5 nm
FI filters
(top)
< 0.15 pM (< 3 amol/well FITC, 384sv, 20 μL).
FI filters
(bottom)
< 1.0 pM (< 50 amol/well FITC, 384g, 50 μL).
FI monochromator
(top)
< 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL).
FI monochromator
(bottom)
< 3.0 pM (< 150 amol/well FITC, 384g, 50 μL).
Dynamic Range: 8 decades in a single measurement.
FP
< 0.5 mP SD at 1 nM FITC (384sv, 20 μL).
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL).
Delta F > 880% (High Calibrator).
Delta F > 30% (Low Calibrator).
TRF
< 20 fM europium, 384, 80 μL (<1.6 amol/well).
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL).
Dynamic range: 8 decades in a single measurement.
AlphaScreen® with laser.
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL).
ABS with spectrometer.
Selectable spectral resolution: 1, 2, 5, and 10 nm.
OD range: 0 to 4 OD; photometric resolution: 0.001 OD.
Accuracy: < 1% at 2 OD.
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD.
Linearity: ≤ 0.8% at 2.0 OD.
Flying mode (1 flash)
8 s (96), 15 s (384), 28 s (1536)
10 flashes
19 s (96), 57 s (384), 3 min 4 s (1536)
Linear, orbital, and double-orbital with user-definable time and speed
+3 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
Multi-user reader control (SMART Control and Voyager) and data analysis software (MARS). Voyager is compatible with CLARIOstar Plus readers from serial number 430-4001.
FDA 21 CFR Part 11 compliance available with SMART Control and MARS.
Integrated fluorophore library.
Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg
Excitation and emission slides for 4 filters each
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Automatic focal height adjustment (0.1 mm resolution)
Up to 2 built-in reagent injectors
Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Reagent back flushing
Up to two integrated barcode readers
Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
Microplate incubator and shaker
Actively regulates O2 and CO2 - 0.1-20%
Magazines for up to 50 plates - continuous loading feature. Only compatible with SMART Control.
Excitation and emission filters
The CLARIOstar Plus can include all or any combination of features listed below at purchase. Upgrading with additional features is possible at any time
Please contact your local representative for more details or a quote
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
Intelligent microplate incubation
Microplate handling system
Low-volume measurements
Filters for a wide range of applications
Functional Microplate Reader Validation
Enhanced cell-based assays
Fluorescence intensity (incl. FRET)
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlex™
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/vis absorbance spectra
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Spectral scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well scanning
6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Robot compatible
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™
Low-noise photomultiplier tube
Red-shifted photomultiplier tube
CCD spectrometer
Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separates ex & em LVF Monochromators
Optical filters:
Ex and em slides hold 4 filters each
LVF Monochromators + optical filters
Use one for ex and the other for em
UV/Vis absorbance spectrometer:
Full spectra or 8 distinct wavelengths in < 1 sec/well
Filters
FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT)
LUM: 240 - 740 nm
LVF Monochromators™
FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT)
LUM: 320 - 740 nm
Linear Variable Dichroic
340 - 740 nm or 340 - 760 nm (red-shifted PMT)
Spectrometer
ABS: 220 - 1000 nm; wavelength precision: ≤ 0.5 nm
FI filters
(top)
< 0.15 pM (< 3 amol/well FITC, 384sv, 20 μL).
FI filters
(bottom)
< 1.0 pM (< 50 amol/well FITC, 384g, 50 μL).
FI monochromator
(top)
< 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL).
FI monochromator
(bottom)
< 3.0 pM (< 150 amol/well FITC, 384g, 50 μL).
Dynamic Range: 8 decades in a single measurement.
FP
< 0.5 mP SD at 1 nM FITC (384sv, 20 μL).
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL).
Delta F > 880% (High Calibrator).
Delta F > 30% (Low Calibrator).
TRF
< 20 fM europium, 384, 80 μL (<1.6 amol/well).
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL).
Dynamic range: 8 decades in a single measurement.
AlphaScreen® with laser.
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL).
ABS with spectrometer.
Selectable spectral resolution: 1, 2, 5, and 10 nm.
OD range: 0 to 4 OD; photometric resolution: 0.001 OD.
Accuracy: < 1% at 2 OD.
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD.
Linearity: ≤ 0.8% at 2.0 OD.
Flying mode (1 flash)
8 s (96), 15 s (384), 28 s (1536)
10 flashes
19 s (96), 57 s (384), 3 min 4 s (1536)
Linear, orbital, and double-orbital with user-definable time and speed
+3 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer
Multi-user reader control (SMART Control and Voyager) and data analysis software (MARS). Voyager is compatible with CLARIOstar Plus readers from serial number 430-4001.
FDA 21 CFR Part 11 compliance available with SMART Control and MARS.
Integrated fluorophore library.
Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg
Excitation and emission slides for 4 filters each
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Automatic focal height adjustment (0.1 mm resolution)
Up to 2 built-in reagent injectors
Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Reagent back flushing
Up to two integrated barcode readers
Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates
Microplate incubator and shaker
Actively regulates O2 and CO2 - 0.1-20%
Magazines for up to 50 plates - continuous loading feature. Only compatible with SMART Control.
Excitation and emission filters
The CLARIOstar Plus can include all or any combination of features listed below at purchase. Upgrading with additional features is possible at any time
Please contact your local representative for more details or a quote
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates
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